Details

Autor(en) / Beteiligte

• Dong, Jing
• Liu, Tao
• Meng, Xiaomeng
• Zhu, Jianying
• Shang, Kun
• Ai, Shiyun
• Cui, Shenglong

Titel

Amperometric biosensor based on immobilization of acetylcholinesterase via specific binding on biocompatible boronic acid-functionalized Fe@Au magnetic nanoparticles

Ist Teil von

• Journal of solid state electrochemistry, 2012-12, Vol.16 (12), p.3783-3790

Ort / Verlag

Berlin/Heidelberg: Springer-Verlag

Erscheinungsjahr

2012

Quelle

SpringerLink

Beschreibungen/Notizen

• A new method based on specific binding between glycoprotein acetylcholinesterase and boronic acid-functionalized Fe@Au magnetic nanoparticles was presented for the development of acetylcholinesterase biosensor. Alginate–graphene composite-modified electrode was firstly prepared as the substrate. Then, biocompatible boronic acid-functionalized Fe@Au magnetic nanoparticles were anchored by the covalence between the cis -diol of alginate and the boronic acid group on Fe@Au nanoparticles. Acetylcholinesterase was subsequently immobilized via the bonding between the glycosyl of acetylcholinesterase and the boronic acid group. The immobilized enzyme retained relatively high bioactivity and the fabricated biosensor exhibited high sensitivity and fast response to acetylthiocholine chloride. Based on enzyme inhibition, carbamate pesticide was detected using Furadan as a model compound. Two linear ranges of 0.05–15 and 15–400 ppb were obtained with a detection limit of 0.01 ppb. The biosensor also showed acceptable reproducibility and relatively good storage stability. Moreover, satisfactory results were obtained in the real sample analysis.