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Direct electrochemistry of glucose oxidase immobilized on porous carbon nanofiber/room temperature ionic liquid/chitosan composite film and its biosensing application
Ist Teil von
Journal of solid state electrochemistry, 2012-02, Vol.16 (2), p.739-745
Ort / Verlag
Berlin/Heidelberg: Springer-Verlag
Erscheinungsjahr
2012
Quelle
Alma/SFX Local Collection
Beschreibungen/Notizen
The direct electron transfer of glucose oxidase (GOD) immobilized on a composite matrix based on porous carbon nanofibers (PCNFs), room-temperature ionic liquid (RTIL), and chitosan (CHIT) underlying on a glassy carbon electrode was achieved. The combination of the PCNFs, RTIL, and CHIT provided a suitable microenvironment for GOD to transfer electron directly. In deaerated buffer solutions (pH 7.0), the cyclic voltammetry of the GOD/PCNFs/RTIL/CHIT composite films showed a pair of well-defined redox peaks with the formal potential of −0.45 V (vs. SCE). The synergistic effort of the PCNFs, RTIL, and CHIT also promoted the stability of GOD in the composite film and retained its bioactivity.