Details

Autor(en) / Beteiligte

• Hultkvist‐Bengtsson, Ursula
• Anderson, Gary P.
• Kings, Madeleine
• Morley, John

Titel

Platelet activating factor‐induced pulmonary accumulation of 111 indium‐oxine labelled neutrophils in anesthetized guinea pigs

Ist Teil von

• Lipids, 1991-12, Vol.26 (12Part2), p.1283-1286

Erscheinungsjahr

1991

Link zum Volltext

Quelle

SpringerLINK Contemporary (Konsortium Baden-Württemberg)

Beschreibungen/Notizen

• Abstract The thoracic accumulation of neutrophils labelled with 111 Indium‐oxine in response to infusion of platelet activating factor (PAF, 18 ng/kg/min × 5 min, i.v.) was studied using an automated isotope monitoring system (AIMSplus) in anesthetized guinea‐pigs. Loss of cell associated radioactivity in vitro was less than 1% over 4 hr. Labelled neutrophils maintained their functional capacity (oxidative response to the cell stimulants N‐formyl‐L‐methionine‐L‐leucine‐L‐phenylalanine and phorbol myristate acetate) and >95% viability (ethidium bromide/acridine orange stain) in vitro . Total thoracic radioactivity increased significantly from baseline in response to PAF with a slight tachyphylaxia in the neutrophil‐accumulation after a repeat PAF infusion. The highest ratios of radiolabel (tissue/blood) were found in the spleen >>liver>lung.