Details

Autor(en) / Beteiligte

• Hu, Lingzhi
• Chen, Junjie
• Yang, Xiaoxia
• Senpan, Angana
• Allen, John S.
• Yanaba, Noriko
• Caruthers, Shelton D.
• Lanza, Gregory M.
• Hammerman, Marc R.
• Wickline, Samuel A.

Titel

Assessing intrarenal nonperfusion and vascular leakage in acute kidney injury with multinuclear 1 H/ 19 F MRI and perfluorocarbon nanoparticles

Ist Teil von

• Magnetic resonance in medicine, 2014-06, Vol.71 (6), p.2186-2196

Erscheinungsjahr

2014

Quelle

Wiley Online Library - AutoHoldings Journals

Beschreibungen/Notizen

• Purpose We sought to develop a unique sensor‐reporter approach for functional kidney imaging that employs circulating perfluorocarbon nanoparticles and multinuclear 1 H/ 19 F MRI. Methods 19 F spin density weighted and T1 weighted images were used to generate quantitative functional mappings of both healthy and ischemia‐reperfusion (acute kidney injury) injured mouse kidneys. 1 H blood‐oxygenation‐level‐dependent (BOLD) MRI was also employed as a supplementary approach to facilitate the comprehensive analysis of renal circulation and its pathological changes in acute kidney injury. Results Heterogeneous blood volume distributions and intrarenal oxygenation gradients were confirmed in healthy kidneys by 19 F MRI. In a mouse model of acute kidney injury, 19 F MRI, in conjunction with blood‐oxygenation‐level‐dependent MRI, sensitively delineated renal vascular damage and recovery. In the cortico‐medullary junction region, we observed 25% lower 19 F signal ( P  < 0.05) and 70% longer 1 H T2* ( P  < 0.01) in injured kidneys compared with contralateral kidneys at 24 h after initial ischemia‐reperfusion injury. We also detected 71% higher 19 F signal ( P  < 0.01) and 40% lower 1 H T2* ( P  < 0.05) in the renal medulla region of injured kidneys compared with contralateral uninjured kidneys. Conclusion Integrated 1 H/ 19 F MRI using perfluorocarbon nanoparticles provides a multiparametric readout of regional perfusion defects in acutely injured kidneys. Magn Reson Med 71:2186–2196, 2014. © 2013 Wiley Periodicals, Inc.