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Analytical subcellular distribution of cAMP-dependent protein kinase activity in bull sperm
Ist Teil von
Gamete research, 1984, Vol.10 (4), p.433-444
Ort / Verlag
Hoboken: Wiley Subscription Services, Inc., A Wiley Company
Erscheinungsjahr
1984
Quelle
Alma/SFX Local Collection
Beschreibungen/Notizen
The distribution of the cAMP‐dependent protein kinase activity in bull ejaculated sperm has been investigated. This activity proved to be mainly present in a soluble form inside the cell. Sperm fractionation into heads and flagellar fragments, using differential centrifugation or centrifugal elutriation, has shown that the particulate cAMP‐dependent protein kinase activity was mainly associated with the flagellar structures. A much activity was shown to be associated with the head fraction. Some activity could also be detected in the purified plasma membrane fraction.