UNIVERSI
TÄ
TS-
BIBLIOTHEK
P
ADERBORN
Anmelden
Menü
Menü
Start
Hilfe
Blog
Weitere Dienste
Neuerwerbungslisten
Fachsystematik Bücher
Erwerbungsvorschlag
Bestellung aus dem Magazin
Fernleihe
Einstellungen
Sprache
Deutsch
Deutsch
Englisch
Farbschema
Hell
Dunkel
Automatisch
Sie befinden Sich nicht im Netzwerk der Universität Paderborn. Der Zugriff auf elektronische Ressourcen ist
gegebenenfalls
nur via VPN oder Shibboleth (DFN-AAI) möglich.
mehr Informationen...
Universitätsbibliothek
Katalog
Suche
Details
Zur Ergebnisliste
Ergebnis 24 von 338
Datensatz exportieren als...
BibTeX
Eupatilin inhibits T-cell activation by modulation of intracellular calcium flux and NF-κB and NF-AT activity
Journal of cellular biochemistry, 2009-09, Vol.108 (1), p.225-236
Kim, Young-Dae
Choi, Suck-Chei
Oh, Tae-Young
Chun, Jang-Soo
Jun, Chang-Duk
2009
Details
Autor(en) / Beteiligte
Kim, Young-Dae
Choi, Suck-Chei
Oh, Tae-Young
Chun, Jang-Soo
Jun, Chang-Duk
Titel
Eupatilin inhibits T-cell activation by modulation of intracellular calcium flux and NF-κB and NF-AT activity
Ist Teil von
Journal of cellular biochemistry, 2009-09, Vol.108 (1), p.225-236
Ort / Verlag
Hoboken: Wiley Subscription Services, Inc., A Wiley Company
Erscheinungsjahr
2009
Link zum Volltext
Quelle
Wiley Blackwell Single Titles
Beschreibungen/Notizen
Eupatilin, one of the pharmacologically active ingredients of Artemisia princeps, exhibits a potent anti‐ulcer activity, but its effects on T‐cell immunity have not been investigated. Here, we show that eupatilin has a profound inhibitory effect on IL‐2 production in Jurkat T cells as well as in human peripheral blood leukocytes. Eupatilin neither influenced clustering of CD3 and LFA‐1 to the immunological synapse nor inhibited conjugate formation between T cells and B cells in the presence or absence of superantigen (SEE). Eupatilin also failed to inhibit T‐cell receptor (TCR) internalization, thereby, suggesting that eupatilin does not interfere with TCR‐mediated signals on the membrane proximal region. In unstimulated T cells, eupatilin significantly induced apoptotic cell death, as evidenced by an increased population of annexin V+/PI+ cells and cleavage of caspase‐3 and PARP. To our surprise, however, once cells were activated, eupatilin had little effect on apoptosis, and instead slightly protected cells from activation‐induced cell death, suggesting that apoptosis also is not a mechanism for eupatilin‐induced T‐cell suppression. On the contrary, eupatilin dramatically inhibited I‐κBα degradation and NF‐AT dephosphorylation and, consequently, inhibited NF‐κB and NF‐AT promoter activities in PMA/A23187‐stimulated T cells. Interestingly, intracellular calcium flux was significantly perturbed in cells pre‐treated with eupatilin, suggesting that calcium‐dependent cascades might be targets for eupatilin action. Collectively, our results provide evidence for dual regulatory functions of eupatilin: (1) a pro‐apoptotic effect on resting T cells and (2) an immunosuppressive effect on activated T cells, presumably through modulation of Ca2+ flux. J. Cell. Biochem. 108: 225–236, 2009. © 2009 Wiley‐Liss, Inc.
Sprache
Englisch
Identifikatoren
ISSN: 0730-2312
eISSN: 1097-4644
DOI: 10.1002/jcb.22244
Titel-ID: cdi_crossref_primary_10_1002_jcb_22244
Format
–
Schlagworte
apoptosis
,
calcium
,
eupatilin
,
IL-2
,
immunosuppression
,
T-cell activation
Weiterführende Literatur
Empfehlungen zum selben Thema automatisch vorgeschlagen von
bX