Details

Autor(en) / Beteiligte

• Grenadyorov, Alexander S
• Solovyev, Andrey A
• Oskomov, Konstantin V
• Semenov, Vjacheslav A
• Zhulkov, Maksim O
• Sirota, Dmitriy A
• Chernyavskiy, Alexander M
• Karmadonova, Nataly A
• Malashchenko, Vladimir V
• Litvinova, Larisa S
• Khaziakhmatova, Olga G
• Gazatova, Natalia D
• Khlusov, Igor A

Titel

Morphofunctional reaction of leukocytes and platelets in in vitro contact with a-C:H:SiO x -coated Ti-6Al-4V substrate

Ist Teil von

• Journal of biomedical materials research. Part A, 2023-03, Vol.111 (3), p.309-321

Ort / Verlag

United States

Erscheinungsjahr

2023

Link zum Volltext

Quelle

Wiley-Blackwell Journals

Beschreibungen/Notizen

• The article deals with the plasma-assisted chemical vapor deposition of 0.3-1.4 μm thick a-C:H:SiO films in a mixture of argon and polyphenylmethylsiloxane vapor onto the Ti-6Al-4V alloy substrate, which is often used as an implant material. The a-C:H:SiO film structure is studied by the Fourier-transform infrared and Raman spectroscopies. The pull-off adhesion test assesses the adhesive strength of a-C:H:SiO films, and the ball-on-disk method is employed to measure their wear rate and friction coefficient. According to these studies, a-C:H:SiO films are highly adhesive to the Ti-6Al-4V substrate, have low (0.056) friction coefficient and wear rate (9.8 × 10  mm  N  m ) in phosphate-buffered saline at 40°C. In vitro studies show neither thrombogenicity nor cytotoxicity of the a-C:H:SiO film for the human blood mononuclear cells (hBMNCs). The in vitro contact between the hBMNC culture and a-C:H:SiO films 0.8-1.4 μm thick deposited onto Ti-6Al-4V substrates reduces a 24-hour secretion of pro-inflammatory cytokines and chemokines IL-8, IL-17, TNFα, RANTES, and MCP-1. This reduction is more significant when the film thickness is 1.4 μm and implies its potential anti-inflammatory effect and possible application in cardiovascular surgery. The dependence is suggested for the concentration of anti-inflammatory cytokines and chemokines and the a-C:H:SiO film thickness, which correlates with the surface wettability and electrostatic potential. The article discusses the possible applications of the anti-inflammatory effect and low thrombogenicity of a-C:H:SiO films in cardiovascular surgery.