Sie befinden Sich nicht im Netzwerk der Universität Paderborn. Der Zugriff auf elektronische Ressourcen ist gegebenenfalls nur via VPN oder Shibboleth (DFN-AAI) möglich. mehr Informationen...
Cu I ‐Catalyzed Azide–Alkyne Intramolecular i ‐to‐( i +4) Side‐Chain‐to‐Side‐Chain Cyclization Promotes the Formation of Helix‐Like Secondary Structures
Ist Teil von
European journal of organic chemistry, 2010-01, Vol.2010 (3), p.446-457
Erscheinungsjahr
2010
Link zum Volltext
Quelle
Alma/SFX Local Collection
Beschreibungen/Notizen
Abstract
A solid‐phase assembly of model peptides derived from human parathyroid hormone‐related protein (11–19) containing ω‐azido‐ and ω‐yl‐α‐amino acid residues in positions
i
and
i
+4 was cyclised in solution by an intramolecular Cu
I
‐catalyzed azide–alkyne 1,3‐dipolar Huisgen cycloaddition. These series of heterodetic cyclo‐nonapeptides varied in the size of the disubstituted 1,2,3‐triazolyl‐containing bridge, the location and the orientation of the 1,2,3‐triazolyl moiety within the bridge. The 1,2,3‐triazolyl moiety, presented at either 1,4‐ or 4,1‐orientation, is flanked by side chains containing 1–4 CH
2
groups that result in bridges comprised from 4–7 CH
2
groups connecting residues 13 and 17. Comprehensive conformational analysis employing CD, NMR and molecular dynamics reveals the conformational propensities of these heterodetic cyclo‐nonapeptides. Cyclo‐nonapeptides containing either the 7 methylene bridge (
VII
and
VIII
) or the 4 methylene bridge (
II
) are unstructured in structure‐promoting solvent. Cyclo‐nonapeptide
I
in which the 1,4‐disubstituted 1,2,3‐triazolyl is flanked by 3 and 1 CH
2
groups in proximity to the respective residues 13 and 17, is stabilized in a non‐canonical structure. All the other heterodetic cyclo‐nonapeptides (
III
–
VI
) in which the 1,2,3‐triazolyl is flanked by a total of 5 or 6 CH
2
groups nicely accommodate α‐helical structures and reproduce very closely the helical structure stabilized by the analogous cyclo‐nonapeptide in which Lys
13
and Asp
17
are bridged by the isosteric lactam. These studies suggest that the bioorthogonal
i
‐to‐(
i
+4) side‐chain‐to‐side‐chain cyclization via the prototypic “click reaction” offers a new and powerful approach for generating stable helix mimetic structures.