Details

Autor(en) / Beteiligte

• Zoorob, Rima
• Bernot, Alain
• Renoir, Dominique Martel
• Choukri, Fatima
• Auffray, Charles

Titel

Chicken major histocompatibility complex class II B genes: analysis of interallelic and inter‐locus sequence variance

Ist Teil von

• European journal of immunology, 1993-05, Vol.23 (5), p.1139-1145

Ort / Verlag

Weinheim: WILEY‐VCH Verlag GmbH

Erscheinungsjahr

1993

Quelle

Wiley Online Library - AutoHoldings Journals

Beschreibungen/Notizen

• Five different chicken B‐LB genes were cloned and sequenced. The comparison of these sequences shows that they can be classified as members of two different families, the B‐LBII family (containing the B‐LBI and B‐LBII genes) and the B‐LBIII family (containing the B‐LBIII, B‐LBIV, and B‐LBV genes). The extent of polymorphism within each of these families was assessed by in vitro amplification of DNA fragments encompassing exon 2 in several haplotypes. The nucleotide sequences were determined, and pairwise relationships were evaluated. In the course of this work, a sixth gene termed B‐LBVI was identified, defining a third family (B‐LBVI family). Polymorphism of the B‐LBIII or B‐LBVI families is far less extensive than that of the B‐LBII family. In this latter, the distribution of conserved and polymorphic residues is similar to what has been described in mammals. These families seem to have been generated by gene duplication events giving rise to several isotypes, as observed in mammals. However, phylogenetic analyses indicate that these families are not homologous to their mammalian counterparts. Evaluation of the level of transcription of these different genes showed that genes from the B‐LBII family are predominantly transcribed over those of the other families.