Details

Autor(en) / Beteiligte

• Gabrielli, Martina
• Tozzi, Francesca
• Falcicchia, Chiara
• D'Arrigo, Giulia
• Amoretti, Stefano
• Joshi, Pooja
• Tonoli, Elisa
• Rutigliano, Grazia
• Masini, Greta
• Nardi, Gabriele
• Battocchio, Elisabetta
• Zenatelli, Rossella
• Radeghieri, Annalisa
• Verderio‐Edwards, Elisabetta
• Ratto, Gianmichele
• Arancio, Ottavio
• Prada, Ilaria
• Origlia, Nicola
• Verderio, Claudia

Titel

Role of microglial extracellular vesicles in the propagation of early synaptic dysfunction in Alzheimer’s disease

Ist Teil von

• Alzheimer's & dementia, 2023-12, Vol.19 (S13), p.n/a

Erscheinungsjahr

2023

Quelle

Wiley Online Library

Beschreibungen/Notizen

• Background Synaptic dysfunction is an early mechanism in Alzheimer’s disease (AD) which involves progressively larger areas of the brain over time. However, how it starts and propagates is unknown. We hypothesised that large extracellular vesicles (EVs) released by microglia may be responsible for these early events in AD. Method Large EVs carrying Aβ peptides (Aβ‐EVs) were obtained from primary microglia exposed to Aβ42. Optical manipulation was combined with time lapse imaging to study EV‐neuron interaction. Then, EVs were stereotaxically injected into naïve mice entorhinal cortex (EC), a vulnerable region in AD, to evaluate: i) long‐term potentiation (LTP) in EC and its main target region, the dentate gyrus of the hippocampus (DG), through field potential extracellular recordings in slices; ii) possible alterations in cortico‐hippocampal network activity by chronic EEG recordings; iii) progressive memory impairment in behavioural tasks. Finally, taking advantage of high‐resolution accurate‐mass spectrometry SWATH™‐MS, we investigated the molecular differences between Aβ‐ and ctrl‐EVs (from cells not exposed to Aβ). The influence of mesenchymal stem cell (MSC) indirect co‐colture with microglia primed with Aβ on cell phenotype, EVs and functions is also being explored. Result Aβ‐EVs were able to move along the axonal surface more efficiently than ctrl‐EVs and predominantly in an anterograde direction, altering dendritic spine morphology in vitro. LTP was impaired in the EC 1h after Aβ‐EV (but not ctrl‐EV) local injection, while 24h later LTP was impaired also in the DG, indicating a spreading of synaptic dysfunction between connected regions. This translated into network alterations and progressive memory deficits. Interestingly, Aβ42 alone was unable to spread alterations. Aβ‐EVs limited in their motion could neither propagate impairment nor cause network abnormalities, thus implicating Aβ‐EV extracellular motion in these processes. Proteomic analysis provided insights into the molecular mechanisms underneath Aβ‐EV motion at the neuronal surface. Conclusion Our data provide evidence of the involvement of microglial EVs in early synaptic dysfunction in AD, through a new mechanism ‐ extracellular motion at the neuronal surface ‐ not implying EV internalization, paving the way for novel therapeutic strategies. Reference: Gabrielli et al., Brain, 2022 https://doi.org/10.1093/brain/awac083 Fundings: 2018‐AARF‐588984, Horizon2020#874721PREMSTEM