Details

Autor(en) / Beteiligte

• Fan, Jin-Xuan
• Peng, Meng-Yun
• Wang, He
• Zheng, Hao-Ran
• Liu, Zong-Lin
• Li, Chu-Xin
• Wang, Xia-Nan
• Liu, Xin-Hua
• Cheng, Si-Xue
• Zhang, Xian-Zheng

Titel

Engineered Bacterial Bioreactor for Tumor Therapy via Fenton-Like Reaction with Localized H 2 O 2 Generation

Ist Teil von

• Advanced materials (Weinheim), 2019-04, Vol.31 (16), p.e1808278

Ort / Verlag

Germany

Erscheinungsjahr

2019

Quelle

Wiley Online Library

Beschreibungen/Notizen

• Synthetic biology based on bacteria has been displayed in antitumor therapy and shown good performance. In this study, an engineered bacterium Escherichia coli MG1655 is designed with NDH-2 enzyme (respiratory chain enzyme II) overexpression (Ec-pE), which can colonize in tumor regions and increase localized H O generation. Following from this, magnetic Fe O nanoparticles are covalently linked to bacteria to act as a catalyst for a Fenton-like reaction, which converts H O to toxic hydroxyl radicals (•OH) for tumor therapy. In this constructed bioreactor, the Fenton-like reaction occurs with sustainably synthesized H O produced by engineered bacteria, and severe tumor apoptosis is induced via the produced toxic •OH. These results show that this bioreactor can achieve effective tumor colonization, and realize a self-supplied therapeutic Fenton-like reaction without additional H O provision.