Details

Autor(en) / Beteiligte

• ZHAO ShuangShuang ZHAO YanXiu GUO Yan

Titel

14-3-3λprotein interacts with ADF1 to regulate actin cytoskeleton dynamics in Arabidopsis

Ist Teil von

• 中国科学：生命科学英文版, 2015 (11), p.1142-1150

Erscheinungsjahr

2015

Link zum Volltext

Quelle

SpringerLink Journals (MCLS)

Beschreibungen/Notizen

• Actin cytoskeleton dynamics is critical for variety of cellular events including cell elongation, division and morphogenesis, and is tightly regulated by numerous groups of actin binding proteins. However it is not well understood how these actin binding proteins are modulated in a physiological condition by their interaction proteins. In this study, we describe that Arabidopsis 14-3-3 λ protein interacted with actin depolymerizing factor 1 （ADF1） in plant to regulate F-actin stability and dynamics. Loss of 14-3-3 λ in Arabidopsis resulted in longer etiolated hypocotyls in dark and changed actin cytoskeleton architecture in hy- pocotyl cells. Overexpression of ADF1 repressed 14-3-3 λ mutant hypocotyl elongation and actin dynamic phenotype. In addi- tion, the phosphorylation level of ADF1 was increased and the subcellular localization of ADF1 was altered in 14-3-3 λ mutant. Consistent with these observations, the actin filaments were more stable in 14-3-3 λ mutant. Our results indicate that 14-3-3 λprotein mediates F-actin dynamics possibly through inhibiting ADF1 phosphorylation in vivo.