Details

Autor(en) / Beteiligte

• DU Ya Rong PAN Dong CHEN Ya Xiong XUE Gang RENZhenXin LI Xiao Man ZHANG Shi Chuan HU Bu Rong

Titel

Irradiation Response of Adipose-derived Stem Cellsunder Three-dimensional Culture Condition

Ist Teil von

• 生物医学与环境科学：英文版, 2015, Vol.28 (8), p.549-557

Erscheinungsjahr

2015

Quelle

Elektronische Zeitschriftenbibliothek (Open access)

Beschreibungen/Notizen

• Objective Adipose tissue distributes widely in human body. The irradiation response of the adipose cells in vivo remains to be investigated. In this study we investigated irradiation response of adipose-derived stem cells （ASCs） under three-dimensional culture condition. Methods ASCs were isolated and cultured in low attachment dishes to form three-dimensional （3D） spheres in vitro. The neuronal differentiation potential and stem-liked characteristics was monitored by using immunofluoresence staining and flow cytometry in monolayer and 3D culture. To investigate the irradiation sensitivity of 3D sphere culture, the fraction of colony survival and micronucleus were detected in monolayer and 3D culture. Soft agar assays were performed for measuring malignant transformation for the irradiated monolayer and 3D culture. Results The 3D cultured ASCs had higher differentiation potential and an higher stem-like cell percentage. The 3D cultures were more radioresistant after either high linear energy transfer （LET） carbon ion beam or low LET X-ray irradiation compared with the monolayer cell. The ASCs＇ potential of cellular transformation was lower after irradiation by soft agar assay. Conclusion These findings suggest that adipose tissue cell are relatively genomic stable and resistant to genotoxic stress.