Details

Autor(en) / Beteiligte

• FU Fang LIU Xiong Lu Bo QU Guoyi ZHENG Hua HUANG Zhijun XU Peihu XU Haixing YIN Yihua LIU Xia ZHANG Xueqiong

Titel

Synthesis and Evaluation of Novel Chitosan Derivatives for Gent Delivery

Ist Teil von

• 武汉理工大学学报：材料科学英文版, 2015 (4), p.852-858

Erscheinungsjahr

2015

Quelle

SpringerLink

Beschreibungen/Notizen

• A series of novel water soluble chitosan derivatives as gene vectors was synthesized. The delivery systems were tested for their ability to form complexes with plasmid DNA by utilizing agarose gel electrophoresis, particle size analysis, zeta potential measurement and scanning electron microscopy. Furthermore, cytotoxicity of chitosan derivatives and transfection efficiency of polyplexes were evaluated in vitro. The experimental results showed that the novel chitosan derivatives had lower cytotoxicity, good DNA condensation, and higher transfection efficiencies compared to chitosan in both 293T and HeLa cell lines. It was indicated that these chitosan derivatives were promising candidates for non-viral gene vectors.