农业科学与技术：A, 2013, Vol.3 (12), p.989-999
2013
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- Autor(en) / Beteiligte
- Titel
- Analysis of Oleanolic Acid and Ursolic Acid, Potential Antifertility Agents in Moringa （Moringa oleifera） Seed
- Ist Teil von
- 农业科学与技术：A, 2013, Vol.3 (12), p.989-999
- Erscheinungsjahr
- 2013
- Quelle
- Alma/SFX Local Collection
- Beschreibungen/Notizen
- Oleanolic acid （OA） and ursolic acid （UA） are isomeric tritcrpenes that are difficult to separate chromatographically. Studies have indicated that both OA and UA have antifertility properties in several livestock species, suppressing attainment of puberty, gonad function and gamete production. Being able to determine the presence and quantity of the OA and UA in moringa and pawpaw seeds will allow for calculation of optimal inclusion levels of these acids in animal diets for antifertility-activity. The purpose of the study was thus to quantify the levels of OA and UA in Moringa and pawpaw seed powder by means of ultra-high performance liquid chromatography with electrospray ionization tandem mass spectrometry （UHPLC-ESI-MS/MS）. Only OA was detected in moringa seeds at a concentration level of 0.508 p.g/g ＋ 0.032 pg/g dry weight. UA and OA could not be detected in pawpaw seeds used in this study. The presence of OA in moringa seeds suggest that the seeds can be included in tilapia diets as a potential antifert