Details

Autor(en) / Beteiligte

• LIU Li na LUO Qing ping CHENG GUO fu HUXue ying YANGJun ZHANGLin SHAOHua bin LI Shao Wen

Titel

solation of Three Strains Subgroup J and gp85 Gene of Avian Leukosis Virus Sequence Analysis

Ist Teil von

• 动物与饲料科学：英文版, 2013 (1), p.11-13

Erscheinungsjahr

2013

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• [Objective] To isolate three strains of avian leukosis virus subgroup J（ALV -J） , and then amplify and sequence the gp85 gene. [ Method] Three strains of ALV- J were isolated from Hubei Province, which were identified by pathological anatomy, DF- 1 cell culture and RT- PCR. And then they were named HB1002, HB1003 and HB1009, respectively. [ Result] Test sequence analysis showed, the length of gp85 gene was 921 bp, consistent with expect result; the nucleotide homology between the three isolates was in 97.7% -99.7% ,the homology of amino acid was in 95.1% - 99%. The nucleotide homology between HPRS - 103 and the three isolates was in the 94.1% - 94.8% ; and the nucleotide homolo- gy between other ALV -J and the three isolates was in 87.6% -97.3%. The phylogenetic trees analysis showed that the homology of JS09GY6 vi- rus and the three isolates was nearest, in 95.2% -97.3%. [ Conclusion] In the test, the three strains of virus which were isolated were ALV- J.