Details

Autor(en) / Beteiligte

• LI Gang LIN Dong-Hai XIE Xin-Xin QIN Li-Fang WANG Jun-Teng LIU Ke

Titel

Uptake and transport of furanodiene in Caco-2 cell monolayers： a comparison study between furanodiene and furanodiene loaded PLGA nanoparticles

Ist Teil von

• 中国天然药物, 2013, Vol.11 (1), p.49-55

Erscheinungsjahr

2013

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• AIM： Furanodiene （FDE） possesses diverse pharmacological activities with high lipophilicity and poor stability. This study prepared FDE loaded PLGA nanoparticles （FDE-PLGA-NPs） and PEGylated PLGA nanoparticles （FDE-PEG-PLGA-NPs） by the spontaneous emulsion solvent diffusion method to improve the stability and bioavailability of FDE. METHODS： FDE-PLGA-NPs and FDE-PEG-PLGA-NPs were characterized for size and size distribution, surface morphology, zeta-potential and entrapment effi- ciency. The stability of FDE, FDE-PLGA-NPs and FDE-PEG-PLGA-NPs in physiological fluids （PBS and artificial gastrointestinal fluids） was evaluated. In vitro cellular uptake and transport studies were performed using Caco-2 cell monolayers. RESULTS： The size of FDE-PLGA-NPs and FDE-PEG-PLGA-NPs ranged from 110-140 rim, the entrapment efficiencies were 87.3% and 89.2%, respec- tively, and the stabilities were enhanced significantly compared with FDE. FDE-PLGA-NPs and FDE-PEG-PLGA-NPs could be taken up by Caco-2 cells freely and transpo