中国病毒学：英文版, 2011, Vol.26 (5), p.338-346
2011
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- Autor(en) / Beteiligte
- Titel
- Production of CCHF Virus-Like Particle by a Baculovirus-Insect Cell Expression Syste
- Ist Teil von
- 中国病毒学：英文版, 2011, Vol.26 (5), p.338-346
- Erscheinungsjahr
- 2011
- Quelle
- SpringerLink
- Beschreibungen/Notizen
- Crimean-Congo Haemorrhagic Fever Virus （CCHFV） is a tick-born virus of the Nairovirus genus within the Bunyaviridae family, which is widespread and causes high fatality. The nucleocapsid of CCHFV is comprised of N proteins that are encoded by the S segment. In this research, the N protein of CCHFV was expressed in insect cells using a recombinant baculovirus. Under an electron microscope, Virus-Like Particles （VLPs） with various size and morphology were observed in cytoplasmic vesicles in the infected cells. Sucrose-gradient purification of the cell lysate indicated that the VLPs were mainly located in the upper fraction after ultracentrifugation, which was confirmed by Western blot analysis and immuno-electron microscopy （IEM）