Details

Autor(en) / Beteiligte

• Ning Jiang Li Meng Hui-Jun Lu Wei Kang Shuai Peng Wei-Qing Pan Ji-Gang Yin Qi-Jun Chen

Titel

Analysis of var genes cloned from a Plasmodium falcivarum isolate in China

Ist Teil von

• 亚太热带医药杂志：英文版, 2012 (2), p.85-90

Erscheinungsjahr

2012

Quelle

Elsevier ScienceDirect Journals

Beschreibungen/Notizen

• <正>Objective:To analyse the rar gene repertoire and characterise the rhondroitin sulphate A (CSA)-binding activity of the Duffy-binding like(I)BI.) domains encoded by the var2csa gene of a Plasmodium falciparum(P.falciparum) isolate in Hainan Province,China.Methods:The sequences of var DBL1 regions were PCR-amplified,sequenced and the sequence characteristics was bioinformalically analysed.Recombinant proteins encoded by the var2csa genes were expressed and purified.The binding activities of the recombinant proteins to CSA receptor was detected by ELISA assays.Results:Fifty six unique DBI.a sequences were obtained,and the sequences represented similar diversity to the var genes of the genome parasite 3D7.There are two var2csa genes in the P.falciparum isolated from Hainan Province.Unlike in other falciparum parasites such as HB3,the two var2csa genes are more diverged.The receptor-binding capacity of DBL-5εand DBI.-6 e domains of HN var2CSA was studied.Conclusions:This work represented the diversity of rar genes of a P.falciparum isolate in China.