Chinese science bulletin, 2006, Vol.51 (24), p.2986-2992
2006
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- Autor(en) / Beteiligte
- Titel
- Genetic analysis and molecular mapping of a presenescing leaf gene psll in rice （Oryza sativa L.）
- Ist Teil von
- Chinese science bulletin, 2006, Vol.51 (24), p.2986-2992
- Erscheinungsjahr
- 2006
- Beschreibungen/Notizen
- A rice psl1 （presenescing leaf） mutant was obtained from a japonica variety Zhonghua 11 via radiation of ^60Co-γ in M2 generation. Every leaf of the mutant began to wither after it reached the biggest length, while the leaves of the wild variety could keep green for 25--35 d. In this study, genetic analysis and gene mapping were carried out for the mutant identified. The SSR marker analysis showed that the mutant was controlled by a single recessive gene （psl1） located on chromosome 2. Fine mapping of the psl1 locus was conducted with 34 new STS markers developed around psl1 anchored region based on the sequence diversity between Nipponbare and 93-11. The psl1 was further mapped between two STS markers, STS2-19 and STS2-26, with genetic distances of 0.43 and 0.11 cM, respectively, while cosegregated with STS2-25. A BAC contig was found to span the psl1 locus, the region being delimited to 48 kb. This result was very useful for cloning of the psl1 gene.