Details

Autor(en) / Beteiligte

• Horák, Daniel
• Shagotova, Tetiana
• Mitina, Nataliya
• Trchová, Miroslava
• Boiko, Nataliya
• Babič, Michal
• Stoika, Rostyslav
• Kovářová, Jana
• Hevus, Orest
• Beneš, Milan J
• Klyuchivska, Olga
• Holler, Petr
• Zaichenko, Alexander

Titel

Surface-Initiated Polymerization of 2-Hydroxyethyl Methacrylate from Heterotelechelic Oligoperoxide-Coated γ-Fe2O3 Nanoparticles and their Engulfment by Mammalian Cells

Ist Teil von

• Chemistry of materials, 2011-05, Vol.23 (10), p.2637-2649

Ort / Verlag

American Chemical Society

Erscheinungsjahr

2011

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• Novel heterotelechelic poly(N-vinylpyrrolidone) (CPA-PVP-IBMB) oligoperoxide containing anchoring carboxyl group from 4-cyanopentanoic acid (CPA) and peroxide end-group from 1-isopropyl-3(4)-[1-(tert-butyl peroxy)-1-methylethyl]benzene (IBMB) chain transfer agent was synthesized and investigated as a surface-active macroinitiator for tailored functionalization of maghemite (γ-Fe2O3) nanoparticles. CPA-PVP-IBMB initiated polymerization of 2-hydroxyethyl methacrylate (HEMA) in ethanol yielding CPA-PVP-block-poly(2-hydroxyethyl methacrylate) (CPA-PVP-block-PHEMA) copolymer. γ-Fe2O3 nanoparticles obtained by precipitation technique were modified by the CPA-PVP-IBMB macroinitiator and CPA-PVP-block-PHEMA copolymer by “grafting-to” approach to the surface using carboxyl end groups of both polymers. Second method, “grafting-from” approach, consisted in polymerization of HEMA initiated from the surface of CPA-PVP-IBMB-coated γ-Fe2O3 nanoparticles yielding CPA-PVP-IBMB-coated γ-Fe2O3/PHEMA nanoparticles. Tests with mice leukemia L1210 cells confirmed nontoxicity of CPA-PVP-IBMB-coated γ-Fe2O3/PHEMA nanoparticles. CPA-PVP-IBMB-coated γ-Fe2O3/PHEMA nanoparticles were easily engulfed by murine macrophages J774.2 and magnetically separated from cells without phagocytic activity.